RANK, Fc fusion (IgG1), Biotin Labeled (Human) Recombinant

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Catalog #

70822

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Description

Human Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor and TNFRSF11A, (Genbank Accession No. NM_003839), a.a. 30-212, fused at the C-terminus to the Fc portion of human IgG1, with a C-terminal Avi-tag, expressed in a HEK293 cell expression system. This protein is enzymatically biotinylated using AviTagTM technology. MW = 49 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation

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Synonyms

Receptor Activator of NF-KB, TRANCE Receptor, TNFRSF11A, Osteoclast differentiation factor receptor (ODFR)

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Gel Filtration Curve

RANK: RANKL inhibition curve using a TR-FRET assay (#79101). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥71%

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

49 kDa+glycans

Amino Acids

30-212

Genbank #

NM_003839

UniProt #

Q9Y6Q6

Tag(s)

C-terminal Avi-tag™

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

References

1. Annis, A., J. American Chemical Society., 2004 May; 126(4): 15495-15503.

2. Yan, T., J. Cellular Biochemistry. 2001 Aug ; 83(2):320-325.

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and for screening small molecules and antibodies.

Shipping Temperature

-80°C

Notes

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

70822.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

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RANK, Fc fusion (IgG1), Biotin Labeled (Human) Recombinant

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