RANK:RANKL TR-FRET Assay

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Catalog #

79101

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Description

The RANK:RANKL TR-FRET Assay is designed to measure the inhibition of RANK binding to RANKL. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated RANK, RANKL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for one hour. Then, the fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against RANK:RANKL? Check out our Immunotherapy Biochemical Screening Services.

This product has been cited 1 time.

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Synonyms

RANK, RANKL, TNFRSF11A, TNF Receptor Superfamily Member 11a, TNF Superfamily Member 11, TNFSF11, TNF Receptor Superfamily Member 11b, TNFRSF11B

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Product Data Gallery

Product Info

Storage and Usage

Citations1

Assay Kit Format

TR-FRET

Format

96 Reactions:

Catalog Number	Components for 96 Reactions	Amount	Storage
70822	RANK, Fc fusion (IgG1), Biotin Labeled (Human)	5 µg	-80°C	Avoid freeze/ thaw cycles!
71051	RANKL-His	2 µg	-80°C
30017	Anti-His Tb Donor	3 x 10 µl	-20°C
	Dye-labeled Acceptor	3 x 10 µl	-20°C
79311	3x Immuno Buffer 1	4 ml	-20°C
	White, 96-well half-area microtiter plate	1	Room Temp.

384 Reactions:

Catalog Number	Components for 384 Reactions	Amount	Storage
70822	RANK, biotinylated	5 µg	-80°C	Avoid freeze/ thaw cycles!
71051	RANKL-His	2 µg	-80°C
30017	Anti-His Tb Donor	2 x 10 µl	-20°C
	Dye-labeled Acceptor	2 x 10 µl	-20°C
79311	3x Immuno Buffer 1	4 ml	-20°C
	White, non-binding, low volume, 384-well microtiter plate	1	Room Temp.

UniProt #

RANK: Q9Y6Q6; RANKL: O14788

References

1. Annis, A., et al. J. Amer.n Chem. Soc., 2004 May; 126(4): 15495-15503.

2. Yan, T., et al. J. Cell. Biochem. 2001 Aug; 83(2): 320-325.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival

Instructions for Use

See assay kit data sheet for detailed protocol

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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RANK:RANKL TR-FRET Assay

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