Recombinant HEK293 cell line expressing full length human CD155, also known
as poliovirus receptor (PVR) or nectin-like molecule-5 (NECL-5). Expression is confirmed by
Western Blot.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
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Synonyms
PVR, NECL-5, CD155-His
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Product Data Gallery
Expression of CD155 protein in CD155/HEK293 cells validated by flow cytometry
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat.
#LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set
(Lonza, Cat. #LT07-518) was used as a positive control.
Background
CD155 is a transmembrane glycoprotein receptor belonging to the
immunoglobulin superfamily. It is highly expressed on dendritic cells, fibroblasts, endothelial
cells, and multiple tumor cells including ovarian carcinoma, non-small cell lung, glioblastoma,
and colorectal carcinoma. It has a high affinity for a T cell regulatory transmembrane surface
protein called TIGIT (T cell immunoglobulin and ITIM domains), and plays a key role in tumor
cell invasion and migration. Interaction between CD155 and TIGIT causes immunosuppressive
effects on CD4+ and CD8+ T cells, which hinders T cell proliferation and function. CD155 also
interacts with regulatory receptors CD226 (expressed on natural killer (NK) cells, monocytes
and CD4+ T cells) and CD96. While CD155-CD226 engagement activates NK cell cytotoxicity
and T cell response, CD155-CD96 interaction inhibits NK cell function. Due to these opposing
regulatory effects, blockade of CD155-TIGIT and CD155- CD226 interactions are attractive
therapeutic strategies in cancer immunotherapy and autoimmune diseases, respectively.
References
1. Yu, X., et.al. (2009) The Surface Protein TIGIT Suppresses T cell Activation by Promoting the Generation of Mature Immunoregulatory Dendritic Cells. Nat Immunol. 10: 48-57. 2. Bottino, C., et.al. (2003) Identification of PVR (CD155) and Nectin-2 (CD112) as Cell Surface Ligands for the Human DNAM-1 (CD226) Activating Molecule. J Exp Med. 198: 557-567. 3. Sloan, K.E., et.al. (2005) CD155/PVR Enhances Glioma Cell Dispersal by Regulating Adhesion Signaling and Focal Adhesion Dynamics. Cancer Res. 65: 10930-37.
Storage/Stability
Store in liquid nitrogen immediately upon receipt.
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Thaw Medium 1 (BPS, #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin
Growth Medium 1F (BPS, #79540): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 100 µg/ml Hygromycin B
Instructions for Use
See product datasheet for detailed protocol.
Applications
The CD155/HEK293 Stable Cell Line functions as an antigen-presenting cell (APC) to stimulate TIGIT-specific signaling responses in lymphocytes, such as cell proliferation and cytokine release, or with a TIGIT reporter cell line (BPS Cat. #60538).
Shipping Temperature
dry ice
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.