PDE1C (Mouse), GST-tag Recombinant

Catalog #

60012

$455 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Mouse PDE1C (GenBank Accession No. NM_001025568), full length with N-terminal GST-tag, MW= 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

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Synonyms

PDE1C, phosphodiesterase 1C, PDE1-C, PDE

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

PDE1C (Mouse) inhibition curve using a fluorescence polarization assay (#61312). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Mouse

Host Species/Expression System

Sf9 insect cells

Purity

≥70%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol, and 3 mM DTT.

100 kDa

Amino Acids

full length

Specific Activity

≥ 16,650 pmol/min/µg

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Genbank #

NM_001025568

UniProt #

Q64338

Tag(s)

N-terminal GST-tag

References

1. Gong S. et al., Nature 425 (6961), 917-925 (2003).
2. Spence S. et al., Biochem. J. 321 (PT 1), 157- 163 (1997).

Storage/Stability

>6 months at -80°C.

Assay Conditions

10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE1C at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

PDE

Regulatory Status

Research Use Only