PDE1C Assay Kit

Catalog #
60312
$405 *
Size: 96 reactions
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Description

The PDE1C Assay Kit is designed for identification of PDE1C inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1C to the binding agent. The key to the PDE1C Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

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Synonyms
inhibitor screening, assay kit, PDE1C, PDE1-C, PDE
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorescence Polarization
Supplied As
The PDE1C inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE1C enzyme, fluorescently labeled PDE1C substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions.
Format

Assay Kit Components

UniProt #
Q14123
Background
Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is expressed principally in human myocardium. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.
References
Vandeput. F., et al. J. Biol. Chem. 2007; 282(45): 32749-32757.