PDE1C (Mouse) Assay Kit

Catalog #

61312

$405 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Mouse PDE1C Assay Kit is designed for identification of inhibitors of Mouse PDE1C using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by Mouse PDE1C to the binding agent.

The Mouse PDE1C Assay Kit comes in a convenient 96-well format, with purified Mouse PDE1C enzyme, fluorescently labeled substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Mouse PDE1C Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for Mouse PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a sample containing Mouse PDE1C for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.

Need us to run inhibitor screens or profile your compounds against PDE1C (Mouse)? Check out our Phosphodiesterase Screening Services.

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Synonyms

inhibitor screening, assay kit, PDE1C, PDE1-C, PDE

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Fluorescence Polarization

Species

Mouse

Supplied As

The PDE1C (Mouse) Assay Kit comes in a convenient 96-well format, with purified murinePDE1C enzyme, fluorescently labeled PDE1C substrate (cAMP), binding agent, and PDEassay buffer for 100 enzyme reactions.

Format

Components

UniProt #

Q64338

Background

Phosphodiesterases (PDEs) play an important role in the dynamic regulation of cAMP and cAMP signaling. Mouse PDE1C, also known as cAMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility.

Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.

References

Chandrasekaran A, et al., Cell Signal. 2008; 20(1): 139-53.

Storage/Stability

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Notes

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable to measure fluorescence polarization

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

This assay requires a fluorescent microplate reader capable of measuring fluorescence polarization (FP) and equipped with the required parts to read the FP signal. For more information FP technology, visit our Tech Note: FP, assay principles and applications.  

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

PDE

Regulatory Status

Research Use Only

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PDE1C (Mouse) Assay Kit

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