PDE1C TR-FRET Assay Kit
Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is expressed principally in human myocardium. The PDE1C TR-FRET Assay Kit is designed for identification of inhibitors of PDE1C using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. The assay is based on the generation of FAM-labeled nucleotide monophosphates by the PDE1C. These phosphate groups bind to terbium-labeled nanoparticles, resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal at 520 nm. The change in fluorescent intensity can be easily measured using a fluorescence plate reader.
Using this kit, only two simple steps on a microtiter plate are required for the PDE1C activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 30 minutes. Then, fluorescence intensity can be measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against PDE1C? Check out our Phosphodiesterase Screening Services.
| Catalog # | Component | Amount | Storage | |
| 60013 | PDE1C recombinant enzyme | 1 µg | -80°C |
Avoid freeze/ |
| 60200 | FAM-Cyclic-3′, 5′-AMP: 20 µM | 20 µl | -80 °C | |
| 60393 | PDE assay buffer | 25 ml | -20 °C | |
| 60394 | Tb donor | 50 µl | -80 °C | |
| 60390 | Binding Agent | 200 µl | +4 °C | |
| 78422 | Binding Buffer A | 20 ml | +4 °C | |
| 78423 | Binding Buffer B | 20 ml | +4 °C | |
| 79685 | Black, low binding NUNC microtiter plate | 1 | Room. Temp | |
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