PARP1, GST-tag

Catalog #: 80501
Size: 20 µg
$415
Species: Human
Host Species: Sf9 insect cells
MW: 140 kDa
Genbank #: NM_001618
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal GST-tag, MW = 140 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
P09874
Synonym(s):
poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1, PARP-1
Unit Definition:
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Assay Conditions:
Enzyme reaction is conducted using BPS's PARP1 Assay Kit (Catalog #80551) at room temperature for 1 hour.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PARP
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

80501_120206-2.pdf