PARP1, GST-Tag, PAR-Labeled Recombinant
Recombinant human PARP1 (poly-ADP-ribose) polymerase 1), full length, encompassing amino acids 2-1014(end). This construct contains an N-terminal GST-tag followed by a Thrombin Cleavage site. The recombinant protein was affinity purified and PARylated (poly ADP-ribosylated) in vitro.
≥90%
Aqueous buffer solution.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 3 mM DTT
Poly-ADP-ribosylation (PAR) was performed in vitro after protein purification.
PAR homeostasis is regulated by the family of PAR polymerases (PARPs) and PARG (Poly (ADP-ribose) glycohydrolase) in response to cellular stress conditions. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Poly-ADP-ribosylation (termed PARylation) is the addition of linear or branched chains of ADP-ribose. PARP and PARG activity are linked to cellular responses in inflammation, ischemia, stroke, and cancer. PARP inhibitors have been used in cancer treatment with success, leading to synthetic lethality when homologous recombination repair (HRR) is already defective. Further studies of PARP will elucidate how the levels of PARylated proteins contribute to disease and can be modulated to provide therapeutic benefit.
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