NEK7, GST-tag Recombinant

Catalog #

40141

$375 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human NEK7, also known as NIMA Related Kinase 7 (GenBank Accession No. NM_133494), full length, a.a. 1-302 (end), with N-terminal GST-tag, MW=61.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

NEK7, NIMA-related kinase 7, Never In Mitosis-Related Kinase 7

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥72%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT and 20% glycerol

63 kDa

Amino Acids

1–302(end)

Specific Activity

47 pmol/min/µg

Genbank #

NM_133494

UniProt #

Q8TDX7

Tag(s)

N-terminal GST-tag

References

1. Kandli, M., et al. Genomics 68: 187-196 (2000).
2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).

Storage/Stability

>6 months at -80°C.

Assay Conditions

Assay was performed in a 25 µl reaction containing 40 mM Tris, pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA, 100 µM ATP, 0.2 mg/ml Casein and NEK7 for 50 min at 30°C. NEK7 activity was measured using ADP-Glo reagents (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40141.pdf

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