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Description
Complex of NLRP3 and NEK7 proteins. The two proteins were co-expressed and affinity purified.
Recombinant human NLRP3 (NLR family pyrin domain containing 3), encompassing amino acids 134-1036 (end). The construct does not contain the pyrin domain (also called DAPIN domain or PYD), which is involved in PYCARD binding. It contains an N-terminal FLAG-tag followed by an Avi-Tag™.
Recombinant human NEK7 (NIMA related kinase 7), encompassing amino acids 2-302 (end). This construct contains an N-terminal His-tag (7xHis).
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Synonyms
NACHT, LRR and PYD domains-containing protein 3, AII, AVP, FCU, MWS, FCAS, KEFH, CIAS1, FCAS1, NALP3, C1orf7, Caterpillar protein 1.1, CLR1.1, DFNA34, PYPAF1, AGTAVPRL, Cryopyrin, NIMA related kinase 7, Never in mitosis A-related kinase 7
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Product Data Gallery
4-20% SDS-Page Coomassie Staining
Product Info
Storage and Usage
Citations
Construct
NLRP3 (FLAG-Pre-134-1036(end)) / NEK7 (His-Pre-2-302(end))
Host Species/Expression System
HEK293
Supplied As
Aqueous Buffer Solution
MW
NLRP3: 105 kDa; NEK7: 37 kDa
Amino Acids
NLRP3: 134-1036(end); NEK7: 2-302(end)
Genbank #
NLRP3: NM_004895; NEK7: NM_133494
UniProt #
NLRP3: Q96P20; NEK7: Q8TDX7
References
1. He, Y., et al. Nature, 2016 Feb; 530: 354-357.
2. He, Y., et al. Trends in Biochem. Sci. 2016 Dec; 41(12):1012-1021.
Storage/Stability
At least 6 months at –80°C.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Shipping Temperature
-80°C
Warnings
Avoid freeze/thaw cycles.
Scientific Category
Immunotherapy
Regulatory Status
Research Use Only