NLRP3 Knockdown THP-1 Cell Pool

Catalog #

82121

$1,250 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

NLRP3 Knockdown THP-1 Cell Pool is a cell pool of THP-1 human monocytic cells. This cell pool was generated by using NLRP3 Human shRNA Lentivirus (BPS Bioscience #82122) to create a THP-1 cell pool with stable knockdown of NLRP3 (NLR family pyrin domain containing 3). THP-1 cells are the most commonly used model cell line in the study of inflammasome activation as they express high levels of NLRP3 and pro-caspase-1.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

NACHT, LRR and PYD domains-containing protein 3, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1, Cold-induced autoinflammatory syndrome 1 protein, Cryopyrin, PYRIN-containing APAF1-like protein 1

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Product Data Gallery

NLRP3 expression levels in NLRP3 Knockdown THP-1 Cell Pool

Product Info

Storage and Usage

Citations

Host Cell Line

THP-1, human monocyte, suspension

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2M	BPS Bioscience #78181

UniProt #

Q96P20

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

NOD, LRR and pyrin domain containing 3 (NLRP3), also known as NALP3 and cryopyrin, is a pattern recognition receptor (PRR) of the NRL (NOD-like receptor) subfamily. It is involved in detection of microbes, endogenous and exogenous stress signals. It is expressed in macrophages and when bound to PYCARD (adaptor ASC protein) forms a caspase-1 activating complex named NRLP3 inflammasome. NLRP3 detects uric acid and extracellular ATP in damaged, and once activated it leads to an immune response. Upon activation NLRP3 inflammasome releases its inactive form partners, HSP90 and SGT1, and binds to PYCARD and caspase-1. Caspase-1 initiates the processing and release of pro-inflammatory cytokines IL-1β and IL-18 and gasdermin D-mediated pyroptotic cell death. Mutations in NLRP3 are known to cause autoinflammatory and neuroinflammatory diseases, such as Alzheimer’s, Parkinson’s, and prion disease. NLRP3 is the most extensively studied inflammasome protein to date due to its array of activators and aberrant activation in several inflammatory diseases. Studies into its function and inhibition can lead to the development of therapeutic avenues for the treatment of auto-inflammatory diseases.

References

Swanson K., et al., 2019 Nature Reviews Immunology 19:477-489.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

As this is a cell pool and not a cell line, BPS Bioscience cannot guarantee the stability of the genetic modifications over time. Clonal selection can be performed. We recommend freezing cell vials very early on and growing the cells for a limited number of passages. Cells should be cultured using Growth Media, which contain selection antibiotics.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 2M (BPS Bioscience #7818): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 µg/ml of puromycin

Applications

Study the effect of NLRP3 knockdown on cellular function.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only