NEK3, GST-tag Recombinant

Catalog #

40140

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human NEK3, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

HSPK36, MGC29949

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

86 kDa

Amino Acids

full length

Genbank #

NM_002498

UniProt #

P51956

Tag(s)

N-terminal GST-tag

Background

NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a). NEK3 mRNA is detected in all the proliferating cell lines with the amount not changing during the cell cycle (1). During Prolactin receptor signaling, VAV2 is phosphorylated and activated by NEK3. Overexpression of NEK3 in Chinese hamster ovary cells increases cytoskeletal re-organization in response to Prolactin while downregulation of NEK3 expression by siRNA block these effects (2). Prolactin also stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens.

References

1.Kimura, M. et al: Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3). Cytogenet Cell Genet. 2001;95(3-4):177-82.
2.Miller, S.L. et al: Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene. 2007 Jul 12;26(32):4668-78.

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NEK3, GST-tag Recombinant

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