NEK11, GST-tag Recombinant

Catalog #

40139

$410 *

Size: 10 µg

Qty

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Description

Recombinant human NEK11, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

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Synonyms

FLJ23495

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

110 kDa

Amino Acids

full length

Genbank #

NM_024800

UniProt #

Q8NG66

Tag(s)

N-terminal GST-tag

Background

NEK11 is a member of the NEK family and the protein localizes to the nucleoli where it may function with NEK2A in the S-phase checkpoint. NEK2 phosphorylates NEK11 in its C-terminal autoinhibitory domain and elevates NEK11 kinase activity by dissociating the autoinhibitory domain from the N-terminal catalytic domain (1). NEK11 protein appears to play a role in DNA replication and response to genotoxic stress. NEK11 activity is activated in HeLa cells treated with various DNA-damaging agents and replication inhibitors. Activation of NEK11 by genotoxic agents is suppressed by inhibitor of ATR/ATM-mediated signalling indicating that NEK11 has a role in S-phase checkpoint downstream of ATR/ATM signalling (2).

References

1. Noguchi K, et al: Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells. J. Biol. Chem. 279: 32716-32727, 2004.
2. Noguchi K, et al: Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses. J. Biol. Chem. 277: 39655-39665, 2002.

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NEK11, GST-tag Recombinant

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