Mouse CTLA4:B7-1 TR-FRET Assay
The mouse CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of mouse CTLA4 binding to mouse B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated mCTLA4, mB7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
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Fluorescence microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
| Catalog # | Component | Amount | Storage | |
| 79001 | mCTLA4, biotinylated* | 15 µg | -80°C | Avoid freeze/ thaw cycles! |
| 79002 | mB7-1-Flag-Avi-His* | 5 µg | -80°C | |
| 30017 | Anti-His Tb Donor | 2 x 10 µl | -20°C | |
| Dye-labeled Acceptor | 2 x 10 µl | -20°C | ||
| 79311 | 3x Immuno Buffer 1 | 4 ml | -20°C | |
| 79969 | White, non-binding, low volume, 384-well microtiter plate | 1 | Room Temp | |
*The initial concentrations of mCTLA4 and mB7-1 are lot-specific and will be indicated on the tubes containing the protein.
Huxley, P., et al. Chem Biol. 2004 Dec;11(12):1651-8.
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