NAMPT Inhibitor Screening Assay Kit

Catalog #: 71276
Size: 96 reactions
$765
Background:
NAMPT catalyzes the formation of nicotinamide mononucleotide from nicotinamide and 5-phosphoribosyl-1-pyrophosphate (PRPP). It is the rate limiting component in the mammalian NAD biosynthesis pathway. The protein is thought to be involved in many important biological processes, including metabolism, stress response and aging.
Description:
The NAMPT Inhibitor Screening Assay Kit is designed to measure NAMPT activity for screening and profiling applications. The NAMPT assay kit comes in a convenient 96-well format, with purified recombinant NAMPT enzyme, NAMPT assay buffer, NAMPT dilution buffer, ATP, Nicotinamide, PRPP, and Ethanol sufficient for 96 enzyme reactions.
Synonym(s):
Nicotinamide phosphoribosyltransferase protein, NAMPT, PBEF1, visfatin, Pre-B Cell-Enhancing Factor, activity assay kit
Format:

COMPONENTS:

Instructions for use:
See product datasheet for detailed assay protocol.
Storage / Stability:
Up to 6 months from date of receipt, when stored as recommended.
Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Ramsey, K.M., et al. (2009) Science 324: 651-654

Nakahata, Y., et al. (2009) Science 324: 654-657.

Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:

Fluorescent microplate reader

Adjustable micropipettor and sterile tips

Aluminum foil

Scientific Category:
Metabolic Enzymes
Product Type:
Assay Kit
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Product Datasheets

71276.pdf