FAP– CHO K1 Recombinant Cell Line (High, Medium or Low Expression)

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Catalog #

79947

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Description

Recombinant FAP-CHO K1 cell line stably expressing a full length human FAP-alpha protein (Genbank accession No: NM004460.2). Surface expression of FAP was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of FAP expression (High, Medium, or Low) to mimic different stages of cancer target cells with various FAP expression levels.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

FAP-alpha cell line, Fibroblast activation protein alpha cell line, prolyl endopeptidase FAP cells

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Product Data Gallery

Flow cytometry analysis of cell surface expression of FAP in CHO K1 cells

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1

Species

Human

Supplied As

Each vial contains ~ 2 x 10⁶ cells in 1 ml of 10% DMSO in FBS.

Genbank #

NM004460.2

UniProt #

Q12884

Mycoplasma Testing

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.

Background

Fibroblast activation protein alpha (FAP-alpha) belongs to the dipeptidyl peptidase IV (DPP4; CD26) gene family and is a cell-surface serine protease that acts on various hormones and extracellular matrix components. FAP expression under physiological conditions is scarce in healthy adult tissues. But FAP gene is highly upregulated in a wide variety of cancers, and is often used as a marker for pro-tumorigenic stroma. It has also been proposed as a molecular target of cancer therapies, and a great deal of research has gone into design and testing of diverse FAP-targeted treatments.

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3B (BPS Bioscience #79529): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 μg/ml Hygromycin B

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Protease

Regulatory Status

Research Use Only