Protease Screening & Profiling

Accelerate your drug discovery program with Protease Screening and Profiling Services from BPS Bioscience, Inc. Trust us to provide you with high quality data with fast turnaround time. Our team of experts along with our broad services portfolio makes it easy to:

  • Screen for inhibitors/targets using our panel of 32 different assays including Cathepsins, Caspases, Dipeptidyl Peptidases (DPPs), Deubiquitinases, and other Proteases
  • Select from IC50 determination and single point concentrations
  • Receive data within days of compound submission
  • Perform follow-up studies using the same proteins manufactured in-house
  • Get questions answered or project guidance in a time-efficient manner

Biochemical Assays:

Aspartic ProteasesCysteine ProteasesMetalloproteasesSerine Proteases
BACE1 A20 MMP1 DPP3
Caspase-3 Ataxin-3 MMP2 DPP4
Caspase-6 Cathepsin B MMP9 (Q279R) DPP7
Caspase-7 Cathepsin F   DPP8
Caspase-8 Cathepsin L   DPP9
Caspase-9 Cathepsin S   FAP
Renin, activated Cathepsin V   HCV1a (D168V)
  OTUD6Bc   HCV1b
  UCHL1   HCV1b (D168V)
  UCHL3   HCV1b (R155K)
  USP2   HCV1b (R155Q)
USP5 HCV2a
USP7 POP
USP8 Protein C, activated
USP10
USP14

Why Protesase Screening? : Protease signaling pathways regulate a wide range of vital physiological processes, including digestion of food, lysosome and proteasome degradation, apoptosis, blood coagulation, and cell cycle regulation.

The dysregulation of protease activity can lead to pathologies such as arthritis and inflammatory diseases,cardiovascular disorders, cancer, osteoporosis, Alzheimer’s, and other neurological disorders. Several small molecule, protease inhibitor drugs are already on the market and many more are in development. 

Background:

Protease is the name given to a class of enzymes that catalyze protein catabolism by hydrolysis of the peptide bonds linking amino acids together. Proteases have evolved to perform these reactions by numerous different mechanisms and are found in animals, plants, bacteria, archaea, and viruses, highlighting the important role that they play in all forms of life on Earth. Proteases are involved in protein processing, regulation of protein function, apoptosis, viral pathogenesis, digestion, photosynthesis, and numerous other vital processes.

More recently, various industries have begun to take advantage of proteases as an active ingredient in specific products or as a research tool. For example, laundry detergents contain proteases to help break down stains; proteases are often added to bread during the baking process to help soften the final product; and the biotech industry takes advantage of highly specific proteases, such as TEV protease and thrombin, to cleave fusion proteins.

Proteases also offer a valuable target in many therapeutic settings, including Alzheimer’s, cancer, and viral infection. In fact, one of the most widely-used classes of anti-viral therapeutics is protease inhibitors, which are used to treat both HIV and HCV infections.

BPS offers a wide selection of purified proteases, assay kits, fluorogenic substrates, and inhibitors/activators to reach all your research needs. In addition, we also offer protease screening and profiling services if you prefer to take advantage of our screening experience and expertise in this area.

References:

1. Overall, C.M. & Kleifeld, O., Nat. Rev. Canc. 2006; 6: 227-239.
2. Turk, B., Nat. Rev. Drug Disc. 2006; 5: 785-799.
3. Anderson, J. et al., Handbook of Experimental Pharmacology. 2009; 189: 85-110.

  • Fluorescence Intensity

Contact us today for more information about our Protease Screening and Profiling Services. Tailor our services to meet your needs: Learn more about our Custom Services.

Not quite what you’re looking for? Our Custom Assay Development Services allow for flexibility to meet your specific research needs