NAMPT Assay Service
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Target
NAMPT
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Description
Screening and/or profiling inhibitor compounds against NAMPT phosphoribosyltransferase activity in a biochemical assay.
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Synonyms
Nicotinamide phosphoribosyltransferase, NAmPRTase; Nampt, Pre-B-cell colony-enhancing factor 1 (Pre-B cell-enhancing factor), Visfatin, PBEF, PBEF1
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Example Data
*Example only, final data may vary.
Assay Details
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Assay Format
Fluorogenic
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Reference Compounds and IC50
FK-866, 10 nM
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Assay Principle
The NAMPT Inhibitor Screening Assay is a 96-well or 384-well fluorescent based assay designed to measure the activity of NAMPT to produce nicotinamide mononucleotide for screening and profiling applications.
Target Details
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Protein Family
Metabolic Enzyme
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UniProt
P43490
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Background
Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the formation of nicotinamide mononucleotide from nicotinamide and 5-phosphoribosyl-1-pyrophosphate (PRPP). It is the rate limiting enzyme in the mammalian NAD (nicotinamide adenine dinucleotide) biosynthesis pathway. NAMPT is thought to be involved in many important biological processes, including metabolism, stress response and aging. NAMPT is often overexpressed in cancer cells, an adaptation required to sustain the higher energetic need of the cells. In addition, it also plays roles in proliferation, invasion, stemness, angiogenesis, immune regulation and drug resistance of tumors, making it an attractive target candidate for cancer therapy.
Delivery
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Estimated Turnaround
Two to three weeks following delivery of compounds
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Results
Extensive report with raw and analyzed data, graphs, and detailed protocols. Includes positive control for inhibition.
