NAMPT (PBEF1), GST-tag Recombinant

Catalog #

91004

$420 *

Size: 50 µg

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Synonyms

Nicotinamide phosphoribosyltransferase protein, NAMPT, PBEF1, visfatin, pre-B-cell colony-enhancing factor 1, NMPT

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

NAMPT inhibition curve using a fluorogenic assay (#71276-1). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

40 mM Tris pH8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 10 mM GSH, 20% glycerol, 3 mM DTT.

82 kDa

Amino Acids

2–491(end)

Specific Activity

≥1.0 pmol/min/µg

Genbank #

NM_005746

UniProt #

P43490

Tag(s)

N-terminal GST-tag

References

1. Amal B et al., Mol. Cell. Biol. 14 (2)
2. Martin PR. et al., J. Bacteriol. 183 (4)

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl2, 0.1 mM nicotinamide, 0.4 mM PRPP, 2 mM ATP, 30 µg/mL of alcohol dehydrogenase, 10 µg/mL of NMNAT (lot 130419), 1.5% alcohol, 1 mM DTT, 0.02% BSA, and various amount of NAMPT in a final volume of 50 µL. Reactions were incubated at room temperature for 30 min, and the increase in NADH fluorescence (Ex 340 nm/Em 460 nm) was measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of protein binding assays, enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Metabolic Enzymes-Transferase

Regulatory Status

Research Use Only

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NAMPT (PBEF1), GST-tag Recombinant

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