CD123 (IL3Ra) - CHO Recombinant Cell Line (High, Medium, or Low Expression)

Catalog #: 79640-H
Size: 2 Vials
$6,000
Catalog #: 79640-M
Size: 2 Vials
$6,000
Catalog #: 79640-L
Size: 2 Vials
$6,000
Background:
CD123, also known as interleukin 3 receptor alpha (IL3Ra), is a transmembrane protein normally expressed on basophils and plasmacytoid dendritic cells and overexpressed on multiple blood cancers and leukemic hematopoietic stem cells. It normally plays a role in cell proliferation. CAR-Ts and other therapies targeting CD123 are currently being evaluated in clinical trials. CD123 may be especially valuable in treating B cell acute lymphoblastic leukemia patients who have relapsed after CD19 CAR-T treatment.
Description:
Stable, recombinant clonal CHO cell line constitutively expressing full length human CD123 protein (Genbank #NM_002183.2). Surface expression of CD123 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD123 expression to mimic different stages of cancer target cells with various CD123 expression levels.
Synonym(s):
IL13Ra, interleukin-3 receptor
Supplied As:
Each vial contains ~ 2 x 10^6 cells in 1 ml of FBS + 10% DMSO.
Host Cell line:
Chinese Hamster Ovary
Mycoplasma Testing:
This cell line has been screened using the Quick Test Mycoplasma Detection Kit (Biotool.com, #B39032) to confirm the absence of mycoplasma contamination.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186):  Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3B (BPS Bioscience, #79529): Thaw Medium 3 (BPS Bioscience, #60186) plus 500 mg/ml Hygromycin (Thermo Fisher/Life Technologies, #10687010).

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
Useful for screening and validating antibodies against CD123 and CD123 CAR-T for immunotherapy research and drug discovery. Also useful for CD123 binding assays to screen for CD123 ligands.
Notes:

LICENSE DISCLOSURE: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. 

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
CAR T
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

* Required Fields

$6,000

Quality Assurance

Product Datasheets

79640-H.pdf
79640-M.pdf
79640-L.pdf