Anti-CD123 IgG Antibody, Biotin-Labeled

Only %1 left

Catalog #

101140

As low as $355 *

Cat # Size Price*

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Biotinylated anti-CD123 IgG antibody is a purified recombinant antibody that recognizes human CD123 antigen. This antibody has been tested for specific binding affinity to purified human CD123 protein.

●

Synonyms

Cell division cycle protein 123 homolog, Protein D123, HT-1080, PZ32, CDC123, C10orf7, D123

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD123: anti-CD123-Biotin Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Host Species/Expression System

Heavy chain (HC) and Light chain (LC) co-expressed in HEK293

Purity

≥90%

Purification

Protein A affinity

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Total: 150 kDa; HC: 51 kDa; LC: 24 kDa

UniProt #

O75794

Label

This antibody is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

Functional validation: The antibody was validated by measuring anti-CD123 binding to CD123 antigen in ELISA assay. The CD123 protein (BPS Bioscience #101035) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CD123 antibody (BPS Bioscience #101140), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Applications

This product is for research use only. It is not suitable for human diagnostic or therapeutic use.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only