Anti-CD123 Antibody, FITC-Labeled

Only %1 left

Catalog #

101955

As low as $95 *

Cat # Size Price*

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

This anti-CD123 antibody is a human purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for binding to human CD123 on the surface of CD123 CHO Recombinant Cells (BPS Bioscience #79640-H).

●

Synonyms

C10orf7, D123, CDC123, PZ32, HT-1080, Cell division cycle protein 123 homolog

●

Product Data Gallery

Cell surface expression of CD123 in CD123 CHO Cell Line

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatant.

Format

Aqueous buffer solution.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

UniProt #

O75794

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Background

CD123, also known as interleukin 3 receptor alpha (IL3Ra), is a transmembrane protein found in pluripotent progenitor cells and involved in hematopoietic lineage differentiation. It can also be found in basophils, plasmacytoid dendritic cells and dendritic cells. CD123 is overexpressed on leukemic hematopoietic stem cells (HSCs), but not normal HSCs. It has been found in acute myeloid and B-lymphoid leukemias, blastic plasmacytoid dendritic neoplasms (BPDCN) and hairy cell leukemia. This expression profile makes CD123 an attractive target in cancer therapy. CAR-Ts and other therapies targeting CD123 are currently being evaluated in clinical trials.

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million CD123 CHO cells (BPS Bioscience #79640-H) were blocked and stained with 1 µg of anti-CD123 antibody, FITC-labeled (BPS Bioscience #101955) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CD123 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis represents the cell count, while X-axis shows FITC intensity.

Applications

Useful for studying expression of CD123 on the cell surface by flow cytometry. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human CD123. It has not been tested on other species.

Warnings

Do not freeze. Protect from light.

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Anti-CD123 Antibody, FITC-Labeled

PRODUCTS

SERVICES

RESOURCES

COMPANY