Anti-CD123 Antibody, Biotin-Labeled

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Catalog #

101733

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Description

This anti-CD123 antibody is a purified, recombinant human monoclonal antibody that recognizes human CD123. This construct contains an Avi-tag™ in the middle of the Heavy Chain constant region. This recombinant antibody has been tested for specific binding to purified recombinant human CD123 by ELISA.

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Synonyms

Translation initiation factor eIF2 assembly protein, C10orf7, D123, Cell division cycle protein 123 homolog (Protein D123), HT-1080, PZ32

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD123:Anti-CD123 Biotin Binding Assay

Biotin-Avidin Pulldown

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Heavy Chain: 52 kDa; Light Chain: 24 kDa + glycans

Glycosylation

This antibody runs at a higher MW by SDS-PAGE due to glycosylation.

UniProt #

O75794

Label

This antibody is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be >90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Background

CD123, also known as interleukin 3 receptor alpha (IL3Ra), is a transmembrane protein found in pluripotent progenitor cells and involved in hematopoietic lineage differentiation. It can also be found in basophils, plasmacytoid dendritic cells and dendritic cells. CD123 is overexpressed on leukemic hematopoietic stem cells (HSCs), but not normal HSCs. It has been found in acute myeloid and B-lymphoid leukemias, blastic plasmacytoid dendritic neoplasms (BPDCN) and hairy cell leukemia. This expression profile makes CD123 an attractive target in cancer therapy. CAR-Ts and other therapies targeting CD123 are currently being evaluated in clinical trials.

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

The antibody was validated by measuring anti-CD123 binding to CD123 antigen by ELISA. CD123, Avi-His-Tag Recombinant (BPS Bioscience #101035) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 4 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at Room Temperature (RT). After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CD123 antibody (BPS Bioscience #101733), serially diluted in Blocking Buffer 2, was added for 30 minutes at RT. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for binding of CD123 in ELISA and in cellular assays.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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Datasheets/Protocols

101733 Lot 230321

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Anti-CD123 Antibody, Biotin-Labeled

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