TPH2 Inhibitor Screening Assay Kit
Catalog #
72054
$790
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Description
The TPH2 Assay Kit is designed to measure TPH2 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.
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Synonyms
tryptophan 5-hyrdoxylase, TRPH, activity assay kit
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Product Data Gallery
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorogenic
Materials Required But Not Supplied
Microplate reader capable of measuring Fluorescence
Format
Catalog Number |
Component |
Amount |
Storage |
|
71193 | TPH2, His-tag | 30 µg | -80°C | Avoid freeze/ thaw cycles! |
TPH Enzyme Solution | 5 ml | -80°C | ||
TPH Reaction Solution | 6 ml | -80°C | ||
TPH Quench Solution | 1 ml | -20°C | ||
79685 | Black, 96 Well Plate | 1 | Room Temp. |
UniProt #
Q8IWU9
Background
Tryptophan 5-hydroxylase 1 and 2 (TPH1 and TPH2) are enzymes that catalyze the monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP) which is subsequently converted to serotonin. By catalyzing the rate-limiting step in the biosynthesis of serotonin, these enzymes also play key roles in the signaling of other neurotransmitters downstream of serotonin synthesis, like melatonin. Additionally, TPH enzymes may play a role in immune regulation through tryptophan depletion.
References
1. Moran G. R., et al. J. Biol. Chem. 1998; 273:12259-12266.
2. Nowak E. C., et al., J. Exp. Med. 2012; 209:2127-2135.