TPH1, His-tag (Human) Recombinant

Catalog #

71192

$335 *

Size: 50 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human tryptophan 5- hydroxylase 1 (TPH1) also known as TRPH, GenBank Accession No. NM_004179, a.a. 105-420 with an N-terminal His-tag, MW = 37 kDa, expressed in an E. coli cell expression system.

●

Synonyms

tryptophan 5-hyrdoxylase, TRPH

●

Product Data Gallery

6-20% SDS-PAGE Coomassie Staining

Specific Activity

TPH1 inhibition curve using a fluorogenic assay (#72053). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E.coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT and 170 mM imidazole.

37 kDa

Amino Acids

105-420

Genbank #

NM_004179

UniProt #

P17752

Tag(s)

N-terminal His-tag

References

1. Cote, F., et al., Proc. Nat. Acad. Sci. USA. 2003; 100(23): 13525-13530.
2. Walther, D.J., et al., Science. 2003; 299: 76.

Storage/Stability

>6 months at –80°C

Assay Conditions

Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 μM tryptophan, 100 mM ammonium sulfate, 100 μM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH1. Fluorescence signal was measured at Em360nm/Ex300nm.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category

Metabolic Enzyme, Immunotherapy

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

71192_141009-1.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

TPH1, His-tag (Human) Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY