IDO1 Fluorogenic Inhibitor Screening Assay Kit

Catalog #: 72037
Size: 96 reactions
$725
Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description:

The IDO1 Fluorogenic Inhibitor Screening Assay Kit is designed to measure enzyme inhibition of indoleamine 2,3-dioxygenase 1 (IDO1). The IDO1 Fluorogenic Inhibitor Screening Assay Kit is simple to use and detects fluorescence at long wavelengths, which minimizes potential errors due to compound interference. In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hourincubation at room temperature, the fluorescence solution is added and incubated at 37oC for four hours. Activity is measured by reading sample fluorescence at l=510 nm following excitation of the reaction product at l=400 nm.

Synonym(s):
Indoleamine 2,3-dioxygenase 1, IDO
Supplied As:
The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 96 reactions.
Contraindications:
DMSO >0.5%, strong acids or bases, ionic detergents, high salt. Warning: the Fluorescence Solution contains a component that is known to be a skin and eye irritant, use caution and appropriate personal protective equipment when handling this component.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival
Application(s):
Useful for the study of IDO1 enzymology, inhibitor screening, and selectivity profiling.
Reference(s):

Seegers, N., et al. J. Biomol. Screen. 2014. 19(9):1266-74.

Warning(s):

Avoid freeze/thaw cycles.

The Fluorescence Solution contains a component that is known to be a skin and eye irritant, use caution and appropriate personal protective equipment when handling this component.

Scientific Category:
Immunotherapy
Product Type:
Assay Kit
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Product Datasheets

72037.pdf