IL-17RA[Biotin]:IL-17A Inhibitor Screening Assay Kit

Catalog #: 72060
Size: 96 reactions
$995
Background:
IL-17A is a pro-inflammatory cytokine produced by TH17 cells that plays a key role in inflammation, autoimmunity, and host defense. It has been shown to stimulate the production of pro-inflammatory cytokines, namely IL-6 and IL-8, by synoviocytes. Due to its role as a mediator of inflammation, the IL-17 pathway has become a popular target for treating diseases that have a strong inflammatory component such as psoriasis, arthritis, Crohn’s disease, and ankylosing spondylitis.
Description:
The IL-17RA[Biotin]:IL-17A Inhibitor Screening Assay Kit is designed for screening inhibitors of IL-17A:IL-17RA interaction. The IL-17RA[Biotin]-IL-17A Binding Assay Kit comes in a convenient 96-well format, with biotin-labeled IL-17RA, purified IL-17A, streptavidinlabeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled IL-17RA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, IL-17A is coated on a 96-well plate. Next, IL-17RA is incubated with IL-17A on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Synonym(s):
Interleukin 17, Interleukin 17 receptor, CTLA8, IL17
Format:

COMPONENTS:

Instructions for use:
See product datasheet for detailed protocol.
Storage / Stability:
One year from date of receipt when stored as directed.
Application(s):
Great for screening small molecules and antibodies that inhibit the binding of IL-17A to IL-17RA.
Reference(s):

1. Iwakura Y, et al. 2008. Immunol Rev. 226:57-79.
2. Bullens DM, et al. 2013. Clin Dev Immunol. 2013:840315.
3. Ley K, et al. 2006. Immunol Res. 34(3):229-42.

Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
PBS buffer
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Cytokine, Immunotherapy, Cell Surface Receptor
Product Type:
Assay Kit
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Product Datasheets

72060.pdf