IL-10 (Human) Colorimetric ELISA Detection Kit

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Catalog #

79895

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Description

Interleukin-10 is a key immunoregulator during various infections that acts as an anti-inflammatory cytokine by inhibiting the activities of Th1, macrophage and NK cells. The IL-10 (Human) Colorimetric ELISA Detection Kit is designed for detecting and quantifying human interleukin-10 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capturing antibody is coated on a 96-well plate. Next, samples containing IL-10 are incubated on the coated plate followed by detecting the captured IL-10 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

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Product Info

Storage and Usage

Citations

Assay Kit Format

Colorimetric

Supplied As

This kit comes in a convenient 96-well format, with capturing and detection antibodies for IL-10, streptavidin-labeled HRP, blocking buffer, IL-10 standard, and colorimetric HRP substrate for a 96-well plate.

Materials Required But Not Supplied

PBS (Phosphate-buffered saline)
PBST (PBS with 0.05% Tween-20)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used without adding 1N HCl, but sensitivity of the assay will be reduced.

Format

96 Reactions:

Catalog Number	Component	Amount	Storage
	Interleukin-10 capturing antibody (1 mg/ml)	10 µl	-80°C	Avoid freeze/ thaw cycles!
	Interleukin-10 detection antibody, biotinylated	5 µl	-80°C
	Human IL-10 standard (1 μg/ml)	20 µl	-80°C
80611	Streptavidin-HRP	5 µl	+4°C
79743	Blocking Buffer 3	50 ml	+4°C
79651	Colorimetric HRP substrate	10 ml	+4°C
79964	Transparent 96-well microplate	1	+4°C

5x 96 Reactions:

Catalog Number	Component	Amount	Storage
	Interleukin-10 capturing antibody (1 mg/ml)	50 µl	-80°C	Avoid freeze/ thaw cycles!
	Interleukin-10 detection antibody, biotinylated	25 µl	-80°C
	Human IL-10 standard (1 μg/ml)	100 µl	-80°C
80611	Streptavidin-HRP	25 µl	+4°C
79743	Blocking Buffer 3	250 ml	+4°C
79651	Colorimetric HRP substrate	50 ml	+4°C
79898	Transparent 96-well microplate	5x 1	+4°C

UniProt #

P22301

References

Couper, K. N., et al. IL-10: The Master Regulator of Immunity to Infection. J. Immunology, 2008,180(9): 5771-5777.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Cytokine

Regulatory Status

Research Use Only