IL-6 (Human) Colorimetric ELISA Detection Kit

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Catalog #
79820
As low as $180 *

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Description

Interleukin-6 is a cytokine produced in response to infections or tissue damage that plays an important role in inflammation, immune response and hematopoiesis. The Colorimetric Human IL-6 Detection Kit is designed for detecting and quantifying human interleukin-6 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capturing antibody is coated on a 96-well plate. Next, samples containing IL-6 are incubated on the coated plate followed by detecting the captured IL-6 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

Synonyms
il-6 kit, Interleukin 6 kit, CD126 kit, IL6 ELISA Detection Kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Colorimetric
Species
Human
Supplied As
This kit comes in a convenient 96-well format, with capturing and detection antibodies for IL-6, streptavidin-labeled HRP, blocking buffer, IL-6 standard, and colorimetric HRP substrate for a 96-well plate.
Materials Required But Not Supplied

PBS (Phosphate-buffered saline) 
PBST (PBS with 0.05% Tween-20)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used without adding 1N HCl, but sensitivity of the assay will be reduced.

Format
96 reactions:
Catalog # Component  Amount Storage
  Interleukin-6 capturing antibody
(1 mg/ml) 
10 µl  -80°C  Avoid
freeze/
thaw
cycles!
  Interleukin-6 detection antibody,
biotinylated 
5 µl  -80°C 
  Human IL-6 standard (1 µg/ml)  20 µl  -80°C 
80611 Streptavidin-HRP  5 µl  +4°C 
79743 Blocking Buffer 3  50 ml  +4°C 
79651 Colorimetric HRP substrate  10 ml +4°C 
79964 Transparent 96-well microplate  +4°C 
 
 
 
 

















 
 
 
5 x 96 reactions:
Catalog # Component  Amount Storage
  Interleukin-6 capturing antibody
(1 mg/ml) 
5 x 10 µl  -80°C  Avoid
freeze/
thaw
cycles!
  Interleukin-6 detection antibody,
biotinylated 
5 x 5 µl  -80°C 
  Human IL-6 standard (1 µg/ml)  5 x 20 µl  -80°C 
80611 Streptavidin-HRP  5 x 5 µl  +4°C
79743 Blocking Buffer 3  5 x 50 ml  +4°C
79651 Colorimetric HRP substrate  5 x 10 ml +4°C
  Transparent 96-well microplate  5 x 1  +4°C

 

 

 

 

 

 

 

 

 

UniProt #
P05231
References

Tanaka T., et al. IL-6 in Inflammation, Immunity and Disease. Cold Spring Harbor Perspect. Biol. 2014. 6(10): 1-16.