IL-6 (Human) Colorimetric ELISA Detection Kit
Interleukin-6 is a cytokine produced in response to infections or tissue damage that plays an important role in inflammation, immune response and hematopoiesis. The Colorimetric Human IL-6 Detection Kit is designed for detecting and quantifying human interleukin-6 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capturing antibody is coated on a 96-well plate. Next, samples containing IL-6 are incubated on the coated plate followed by detecting the captured IL-6 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
PBS (Phosphate-buffered saline)
PBST (PBS with 0.05% Tween-20)
1N HCl (aqueous)
Rotating or rocker platform
UV/Vis spectrophotometer microplate reader capable of reading absorbance at 450 nm*
*Alternatively, a spectrophotometer reading at 650 nm may be used without adding 1N HCl, but sensitivity of the assay will be reduced.
Catalog # | Component | Amount | Storage | |
Interleukin-6 capturing antibody (1 mg/ml) |
10 µl | -80°C | Avoid freeze/ thaw cycles! |
|
Interleukin-6 detection antibody, biotinylated |
5 µl | -80°C | ||
Human IL-6 standard (1 µg/ml) | 20 µl | -80°C | ||
80611 | Streptavidin-HRP | 5 µl | +4°C | |
79743 | Blocking Buffer 3 | 50 ml | +4°C | |
79651 | Colorimetric HRP substrate | 10 ml | +4°C | |
79964 | Transparent 96-well microplate | 1 | +4°C |
Catalog # | Component | Amount | Storage | |
Interleukin-6 capturing antibody (1 mg/ml) |
5 x 10 µl | -80°C | Avoid freeze/ thaw cycles! |
|
Interleukin-6 detection antibody, biotinylated |
5 x 5 µl | -80°C | ||
Human IL-6 standard (1 µg/ml) | 5 x 20 µl | -80°C | ||
80611 | Streptavidin-HRP | 5 x 5 µl | +4°C | |
79743 | Blocking Buffer 3 | 5 x 50 ml | +4°C | |
79651 | Colorimetric HRP substrate | 5 x 10 ml | +4°C | |
Transparent 96-well microplate | 5 x 1 | +4°C |
Tanaka T., et al. IL-6 in Inflammation, Immunity and Disease. Cold Spring Harbor Perspect. Biol. 2014. 6(10): 1-16.