IL-6:IL-6R Inhibitor Screening Assay Kit
Interleukin 6 (IL-6), and its receptor complex interleukin 6 receptor (IL-6R, CD126)-gp130 (CD130), play an important role in immune response. Elevated IL-6 leads to an acute severe systemic inflammatory response known as ‘cytokine storm’, where an uncontrolled and excessive release of pro-inflammatory cytokines can cause multisystem organ failure and death. Mortality in COVID-19 patients has been linked to this severe response induced by the virus. Tocilizumab is a humanized monoclonal antibody against the IL-6R used as immunosuppressive drug, and it is emerging as an alternative treatment for COVID-19 patients at risk for ‘cytokine storm’.
The IL-6:IL-6R Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this receptor-ligand interaction. The key to this kit is the high sensitivity of detection of IL-6-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, IL-6R protein is attached to a nickel-coated 96-well plate. Next, IL-6-Biotin incubated with IL-6R on the plate. Finally, the plate is treated with Streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.
Need us to run inhibitor screens or profile your compounds against IL-6:IL-6R? Check out our Immunotherapy Biochemical Screening Services.
PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
| Catalog Number |
Component |
Amount |
Storage |
|
| Human IL-6R alpha, Fc Tag (IL-6R) | 10 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
|
| Biotinylated Human IL-6 (IL-6-Biotin) | 2 µg | -80°C | ||
| 79311 | 3x Immuno Buffer 1 | 50 ml | -20°C | |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C | |
| 79742 | Streptavidin-HRP | 10 µl | +4°C | |
79670 |
ELISA ECL substrate A (transparent bottle) | 6 ml | Room Temp | |
| ELISA ECL substrate B (brown bottle) | 6 ml | Room Temp |
||
| 79699 | 96-well white microplate | 1 | +4°C | |
Tanaka, T. et al. 2016. Future Medicine 8(8): 959-970
Zhang, C. et al. 2020. Int.J. Antimicrob. Agents 55(5): 105954.
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