IL-6:IL-6R Inhibitor Screening Assay Kit

Catalog #
78027
$805 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Interleukin 6 (IL-6), and its receptor complex interleukin 6 receptor (IL-6R, CD126)-gp130 (CD130), play an important role in immune response. Elevated IL-6 leads to an acute severe systemic inflammatory response known as ‘cytokine storm’, where an uncontrolled and excessive release of pro-inflammatory cytokines can cause multisystem organ failure and death. Mortality in COVID-19 patients has been linked to this severe response induced by the virus. Tocilizumab is a humanized monoclonal antibody against the IL-6R used as immunosuppressive drug, and it is emerging as an alternative treatment for COVID-19 patients at risk for ‘cytokine storm’.


The IL-6:IL-6R Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this receptor-ligand interaction. The key to this kit is the high sensitivity of detection of IL-6-Biotin protein by Streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, IL-6R protein is attached to a nickel-coated 96-well plate. Next, IL-6-Biotin incubated with IL-6R on the plate. Finally, the plate is treated with Streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.

IL-6R Inhibitor Screening Assay Kit

Need us to run inhibitor screens or profile your compounds against IL-6:IL-6R? Check out our Immunotherapy Biochemical Screening Services.

Synonyms
Interleukin 6 kit, IL6R inhibitor screening, IL6 kit, interleukin 6 receptor inhibitor screening, cytokine assay, IL-6-Biotin
Product Info
Storage and Usage
Citations
Assay Kit Format
Chemiluminescent
Species
Human
Supplied As
This kit comes in a convenient 96-well format, with purified IL-6R and IL-6-Biotin proteins, Streptavidin-HRP, and assay buffer for 100 binding reactions.
Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format
Catalog
Number

Component

Amount

Storage
  Human IL-6R alpha, Fc Tag (IL-6R) 10 µg -80°C








Avoid

multiple
freeze/
thaw
cycles!
  Biotinylated Human IL-6 (IL-6-Biotin) 2 µg -80°C
79311 3x Immuno Buffer 1 50 ml -20°C
79728 Blocking Buffer 2 50 ml +4°C
79742 Streptavidin-HRP 10 µl +4°C



79670
ELISA ECL substrate A (transparent bottle) 6 ml Room Temp
ELISA ECL substrate B (brown bottle) 6 ml Room
Temp
79699 96-well white microplate 1 +4°C
UniProt #
IL-6: P05231; IL-6R: P08887
References

Tanaka, T. et al. 2016. Future Medicine 8(8): 959-970
Zhang, C. et al. 2020. Int.J. Antimicrob. Agents 55(5): 105954.