Hippo Pathway TEAD Reporter - MCF7 Recombinant Cell Line

Catalog #: 60618
Size: 2 vials
The Hippo pathway regulates cell proliferation and cell death. It is activated by high cell density and cell stress to stop cell proliferation and induce apoptosis. The mammalian Hippo pathway comprises MST kinases and LATS kinases. When the Hippo pathway is activated, MST kinases phosphorylate LATS kinases, which phosphorylate transcriptional co-activators YAP and TAZ. Unphosphorylated YAP and TAZ remain in nucleus and interact with TEAD/TEF transcriptional factors to turn on cell cycle-promoting gene transcription. However, when phosphorylated, YAP and TAZ are recruited from the nucleus to the cytosol, so that the YAP and TAZ-dependent gene transcription is turned off. Dysfunction of the Hippo pathway is frequently detected in human cancer and its down-regulation correlates with the aggressive properties of cancer cells and poor prognosis.
The TEAD Reporter – MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line, MCF7. Inside the cells, basal unphosphorylated YAP/TAZ remains in the nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter by the activators of the Hippo pathway.
Materials Required But Not Provided:

Materials Required but Not Supplied for Cell Culture

Materials Required but Not Supplied for Cellular Assay

  • H2O2: activator of Hippo pathway (activate MST kinases)
  • Insulin
  • Assay Medium: Thaw Medium 1 (BPS Cat. #60187)  + 10 µg/ml of insulin (Sigma-Aldrich # I0516)
  • Insulin Solution from Bovine Pancreas (Sigma-Aldrich #: I0516)
  • Okadaic acid (BPS bioscience #27047): activator of Hippo pathway (activate MST kinases). Prepare 10 mM stock in DMSO.
  • 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
  • ONE-Step™ Luciferase Assay System (BPS, Cat. #60690)
  • Luminometer
Hippo, Hippo signalling pathway, Hippo signalling cascade, Salvador-Warts-Hippo signalling pathway, SWH signalling pathway
Supplied As:
Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Host Cell line:
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Bioscience #60187) + 10 µg/ml of Insulin (Sigma-Aldrich # I0516): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01), plus 10 µg/ml of insulin (Sigma-Aldrich # I0516)

Growth Medium 1B (BPS Bioscience #79531) + 10 µg/ml of Insulin (Sigma-Aldrich # I0516): Thaw Medium 1 (BPS Cat. #60187) + 10 µg/ml of insulin (Sigma-Aldrich # I0516), and at first passage add 400 µg/ml of Geneticin (invitrogen #11811031).

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Monitor Hippo pathway activity. Screen for activators or inhibitors of the Hippo pathway.

1. Lamar JM et al. (2012) The Hippo pathway target, YAP, promotes metastasis through its TEAD-interaction domain. Proc Natl Acad Sci U S A. 109(37):E2441-50. doi: 10.1073/pnas.1212021109.
2. Hata Y et al. (2013) Okadaic Acid: a tool to study the hippo pathway. Mar Drugs. 11(3):896-902. doi: 10.3390/md11030896.
3.. Bao Y et al. (2011) A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription. J Biochem. 150(2):199-208.

Application Reference(s):
1. A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis (2017)


License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles.
Product Type:
Cell Line
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