Hippo Pathway/TEAD Luciferase Reporter MCF7 Cell Line

Catalog #

60618

$2,570 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The TEAD Luciferase Reporter MCF7 cell line contains the firefly luciferase gene under the control of TEAD responsive elements stably integrated into the human breast cancer cell line MCF7. When the Hippo pathway is not stimulated, transcriptional coactivators YAP (Yes-associated protein) and TAZ (also known as WWTR1, WW domain containing transcription regulator 1) are localized in the nucleus and induce constitutive expression of the luciferase reporter. The cell line has been validated for the inhibition of the luciferase reporter by activators of the Hippo pathway, and for the activation of the luciferase reporter by inhibitors of the Hippo pathway.

Interested in screening and profiling activators of the Hippo pathway without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 7 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Hippo, Hippo signalling pathway, Hippo signalling cascade, Salvador-Warts-Hippo signalling pathway, SWH signalling pathway

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Product Data Gallery

Inhibition of TEAD activity in response to H2O2 and Okadaic Acid

Inhibition of TEAD activity in response to Okadaic Acid

Inhibition of TEAD activity in response to H2O2

Activation of TEAD in response to Serum

Product Info

Storage and Usage

Citations7

Host Cell Line

MCF7 cells, human breast mammary gland cell line, epithelial cells, adherent

Species

Human

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1B	BPS Bioscience #79531
Insulin Solution from Bovine Pancreas	Sigma-Aldrich #I0516

Materials Used in the Cellular Assay

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Insulin Solution from Bovine Pancreas	Sigma-Aldrich #I0516
Okadaic acid (stock 10 mM in DMSO)	BPS Bioscience #27047
H₂O₂
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well tissue culture-treated white clear-bottom assay plate
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The Hippo cell signaling pathway regulates organ size and acts as a tumor suppressor by suppressing the transcription of genes controlled by transcription factor TEAD (Transcriptional enhancer factor TEF-1). The Hippo pathway is activated by high cell density and cellular stress to stop cell proliferation and induce apoptosis. Activation of this pathway triggers a kinase cascade in which MST (Macrophage-stimulating) kinases phosphorylate LATS (Large tumor suppressor) kinases, which then phosphorylate transcriptional co-activators YAP and TAZ. In unstimulated cells, dephosphorylated YAP and TAZ are localized in the nucleus and interact with TEAD transcriptional factors to induce cell cycle-promoting gene transcription. Upon stimulation of the Hippo pathway, newly phosphorylated YAP and TAZ are sequestered in the cytosol, and TEAD-dependent gene transcription is turned off. Dysfunction of the Hippo pathway is frequently detected in human cancer and correlates with cancer aggressiveness and poor prognosis.

References

1. Lamar JM et al. (2012) The Hippo pathway target, YAP, promotes metastasis through its TEAD-interaction domain. Proc Natl Acad Sci U S A. 109(37):E2441-50. doi: 10.1073/pnas.1212021109.
2. Hata Y et al. (2013) Okadaic Acid: a tool to study the hippo pathway. Mar Drugs. 11(3):896-902. doi: 10.3390/md11030896.
3. Bao Y et al. (2011) A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription. J Biochem. 150(2):199-208.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

These cells require insulin (10 µg/ml) for optimal growth. Add insulin to the media for cell growth and for cellular assays.

Media Required for Cell Culture

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1B (BPS Bioscience #79531) plus insulin: MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 400 µg/ml Geneticin and 10 µg/ml insulin.

Media Required for Functional Cellular Assay

Thaw Medium 1 (BPS Bioscience #60187) plus insulin: MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin and 10 µg/ml insulin.

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Applications

Monitor Hippo signaling pathway
Screen for activators or inhibitors of the Hippo pathway
Measure the activity of transcription factor TEAD

Shipping Temperature

-80°C (dry ice)

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only