TEAD Luciferase Reporter Lentivirus

Catalog #

79833

$875 *

Size: 500 µl x 2

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The Hippo pathway regulates cell proliferation and cell death. It is activated by high cell density and cell stress to stop cell proliferation and induce apoptosis. The mammalian Hippo pathway comprises MST kinases and LATS kinases. When the Hippo pathway is activated, MST kinases phosphorylate LATS kinases, which phosphorylate transcriptional co-activators YAP and TAZ. Unphosphorylated YAP and TAZ remain in nucleus and interact with TEAD/TEF transcriptional factors to turn on cell cycle-promoting gene transcription. However, when phosphorylated, YAP and TAZ are recruited from the nucleus to the cytosol, so that the YAP and TAZ-dependent gene transcription is turned off. Dysfunction of the Hippo pathway is frequently detected in human cancer and its down-regulation correlates with the aggressive properties of cancer cells and poor prognosis.
The TEAD Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the TEAD response elements located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the Hippo pathway in the target cells can be monitored by measuring the luciferase activity.

Figure 1. Schematic of the lenti-vector used to generate the TEAD luciferase reporter lentivirus.

This product has been cited 9 times.

●

Synonyms

TEAD Luciferase Reporter Lentivirus (Hippo Pathway), MST kinases, LATS kinases

●

Product Data Gallery

Product Info

Storage and Usage

Citations9

Supplied As

Two vials (500 µl x 2) of TEAD luciferase reporter lentivirus at a titer 1 x 10⁷ TU/ml.

The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

• H2O2 (Fisher Scientific # H323-500): activator of Hippo pathway
• Okadaic acid (BPS Bioscience #27047): prepare 10 mM stock in DMSO.
• Insulin Solution from Bovine Pancreas (Sigma-Aldrich #: I0516)
• Assay Medium: Thaw Medium 1 (BPS Bioscience #60187) + 10 µg/ml of insulin
• Polybrene (Millipore, #TR-1003-G)
• 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
• One-Step luciferase assay system (BPS Bioscience, #60690)
• Luminometer

Formulation

The lentiviruses were produced from HEK293T cells in the medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions,

Shipping Temperature

-80°C

Notes

Biosafety:
The lentiviruses are produced with the second generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal.
Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Warnings

Avoid repeated freeze-thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

TEAD Luciferase Reporter Lentivirus

PRODUCTS

SERVICES

RESOURCES

COMPANY