G9a (EHMT2), GST-tag (Sf9-derived) Recombinant

Catalog #

51001

$360 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Human G9a, also known as EHMT2 (euchromatic histone-lysine N-methyltransferase 2) and KMT1C (GenBank Accession No. NM_006709), a.a. 785-1210, with N-terminal GST-tag, MW = 74.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

This product has been cited 3 times.

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Synonyms

G9a, EHMT2, KMT1C, euchromatic histone-lysine N-methyltransferase 2

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

G9a inhibition curve using an AlphaLISA assay (#52051). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations3

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥50%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 Mm KCl, 3 mM DTT and 10% glycerol

74.6 kDa

Amino Acids

785–1210

Specific Activity

≥70 pmol/min/µg

Genbank #

NM_006709

UniProt #

Q96KQ7

Tag(s)

N-terminal GST-tag

Background

EHMT2 (G9a) is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation. EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.

References

1. Yuan,X. et al., Mol. Cell 27, 4, 585-595, 2007.
2. Nishida,M. et al., Genes Cells 12, 7, 877-888, 2007.

Storage/Stability

>6 months at -80°C.

Assay Conditions

50 μl reaction mix (50 mM TRIS pH 8.8, 5mM MgCl2, 4mM DTT, 0.05% Tween-20, 40 μM S- adenosylmethionine, and G9a (0.05 – 0.5 ng) add to the wells coated with the substrate on a GBioscience Neutravidin black plate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only