Anti–G9a polyclonal antibody

Catalog #

25224

$335 *

Size: 100 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Polyclonal antibody raised in rabbit against mouse G9a (Protein G9a), using two KLH–conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

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Synonyms

EHMT2, BAT8, KMT1C, NG36

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Product Info

Storage and Usage

Citations

Clonality

polyclonal

Host Species/Expression System

Rabbit

Purification

Whole serum

Format

aqueous solution

Formulation

Whole antiserum from rabbit containing 0.05% azide

Concentration

100 µl

Immunogen

synthetic peptide

Background

G9a (UniProtKB/Swiss–Prot entry Q96KQ7) is a member of the Set 1 family of histone methyltransferases. It preferentially methylates K9 and K27 of histone H3. Methylation of H3K9 represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9a is a part of the E2F6.com–1 complex.

Storage/Stability

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.

Assay Conditions

Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a (Cat. No. 25224). The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.

Western blot analysis using the antibody directed against G9a
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a (Cat. No. 25224), diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.

Applications

ELISA (1:500 – 1:1000)
WB (1:1000)

Shipping Temperature

-20°C or-80°C

Species Reactivity

Mouse

Notes

The optimal antibody concentration should be determined by the end–user.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Antibodies

Regulatory Status

Research Use Only

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Anti–G9a polyclonal antibody

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