G9a (EHMT2), GST-tag (E. coli-derived) Recombinant

Catalog #

51000

$360 *

Size: 50 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human G9a, also known as EHMT2 (euchromatic histone-lysine N-methyltransferase 2) and KMT1C (GenBank Accession No. NM_006709), a.a. 785-1210, with N-terminal GST-tag, MW = 74.6 kDa, expressed in an E. coli expression system.

This product has been cited 9 times.

●

Synonyms

G9a, EHMT2, KMT1C, euchromatic histone-lysine N-methyltransferase 2

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations9

Species

Human

Host Species/Expression System

E. coli

Purity

≥80%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

74.6 kDa

Amino Acids

785–1210

Specific Activity

22 pmol/min/µg

Genbank #

NM_006709

UniProt #

Q96KQ7

Tag(s)

N-terminal GST-tag

Background

EHMT2 (G9a) is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1) (1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation (2). EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.

References

1. Yuan,X. et al., Mol. Cell 27, 4, 585-595, 2007.
2. Nishida,M. et al., Genes Cells 12, 7, 877-888, 2007.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme was incubated with 0.5 µM of biotinylated H3 peptide with 40 µM AdoMet in HMT buffer 1A in a 10-µl reaction. 5 µl of Anti-Mouse AlphaLISA acceptor beads (1:250) and 5 µl of Primary Antibody 7 (1:50) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 µl) and mixture was further incubated for 15 min at RT before counts were measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only