Furin Protease Assay Kit
The Furin Protease Assay Kit is designed to measure Furin Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.
Need us to run inhibitor screens or profile your compounds against Furin Protease? Check out our Protease Screening Services or Coronavirus Services.
This product has been cited 7 times.
Fluorescent microplate reader capable of reading λexc/λem=380 nm/460 nm
| Catalog # | Component | Amount | Storage | |
| Recombinant Human Furin | 3 µg | -80°C | Avoid freeze/ thaw cycles! |
|
| Furin Protease Substrate (500 μM) | 50 µl | -80°C | ||
| Furin Assay Buffer | 25 ml | -20°C | ||
| 78713 | Chloromethylketone (100 μM) | 20 µl | -80°C | |
| 79685 | Black, low binding microtiter plate | 1 | Room Temp |
|
Furin is a member of the proprotein convertase (PC) family, which belongs to the subtilisin superfamily of serine protease. This protease is thought to cleave and activate more than 150 mammalian, viral and bacterial substrates. Among them are viral envelope glycoproteins and bacterial toxins, as well as cellular factors that can promote tumor development and growth. Cleavage of the SARS coronavirus spike glycoprotein by Furin is a critical step in viral fusion and entry, making furin a potential target for SARS-CoV-2 therapy.
1. Braun, E. and Sauter, D. (2019). Furin‐mediated protein processing in infectious diseases and cancer. Clin Transl Immunol, 8: e1073. doi:10.1002/cti2.1073.
2. Follis, K.E., et al. (2006). Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry. Virology. 350(2):358-369. doi:10.1016/j.virol.2006.02.003
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