Papain-like Protease (SARS-CoV-2) Assay Kit: Protease Activity
The Papain-like Protease Assay Kit: Protease Activity is designed to measure Papain-like Protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.
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This product has been cited 25 times.
Fluorescent microplate reader capable of reading λexc/λem=360 nm/460 nm
96 Reactions
Catalog Number |
Component |
Amount |
Storage |
|
100735 | Recombinant Papain-like Protease, PLPro | 2 µg | -80°C | Avoid freeze/ thaw cycles! |
79997 | PLPro Substrate (5 mM) | 25 µl | -80°C | |
78039 | PLPro Assay Buffer | 25 ml | -20°C | |
10 mM GRL0617 (10 mM) | 20 µl | -80°C | ||
Dithiothreitol (DTT; 0.5 M) | 200 µl | -20°C | ||
79685 | Black, low binding microtiter plate | 1 | Room Temp |
|
Plate sealing film | 1 |
384 Reactions
Catalog Number |
Component |
Amount |
Storage |
|
100735 | Recombinant Papain-like Protease, PLPro | 2 µg | -80°C | Avoid freeze/ thaw cycles! |
79997 | PLPro Substrate (5 mM) | 50 µl | -80°C | |
78039 | PLPro Assay Buffer | 25 ml | -20°C | |
GRL0617 (10 mM) | 20 µl | -80°C | ||
Dithiothreitol (DTT; 0.5 M) | 200 µl | -20°C | ||
79961 | Black, low binding microtiter plate | 1 | Room Temp |
|
Plate sealing film | 2 |
Coronaviruses (CoVs) primarily cause multiple respiratory and intestinal infections in humans and animals. Papain-Like Protease (PLPro), also known as PLP, plays an essential role in polypeptide processing during virus replication. PLProis also proposed to be a key enzyme in the sustained pathogenesis of SARS-CoV-2. PLP acts as a deubiquitinase that removes ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate antiviral immune responses. As a result, PLPro is an important potential target for antiviral drugs that may inhibit viral replication and simultaneously weaken dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells. PLPro inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering with the COVID-19 coronavirus infection.
Weglarz-Tomczak, E. et al., 2020. https://doi.org/10.1101/2020.05.17.100768.