PLPro, His-Tag (SARS-CoV-2) Recombinant

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Catalog #

100735

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Description

Recombinant SARS-Cov-2 papain-like protease protein (PLPro) also known as ofr1ab polyprotein, encompassing amino acids 1564-1882. This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.

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Synonyms

Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein, Papain-like protease nsp3, SARS-CoV, protease, n-CoV 2019 protease, rep, 1a-1b, nsp1, Mpro, SARS coronavirus main proteinase, GFL, Hel, ExoN, Pol, RdRp

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

PLPro Activity

Product Info

Storage and Usage

Citations

Species

SARS-CoV-2

Construct

PLPro (His-1564-1882) (SARS-CoV-2)

Host Species/Expression System

E. coli

Purity

≥80%

Format

Aqueous buffer solution.

Formulation

40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol, and variable Imidazole.

37 kDa

Amino Acids

1564-1882

Genbank #

QHD43415

UniProt #

P0DTD1

Tag(s)

N-terminal His-tag

References

1. Ma-Lauer, Y., et al., PNAS, 2016 Aug; 113(35): E5192-E5201.
2. Mukherjee, S. D., et al., Eu. PMC. 2020 May; DOI: 10.21203/rs.3.rs-27134/v1.

Application Reference(s):
1. An Enzymatic TMPRSS2 Assay for Assessment of Clinical Candidates and Discovery of Inhibitors as Potential Treatment of COVID-19 (2020)

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Assay was done according to Papain-like Protease (SARS-CoV-2) Assay Kit: Protease Activity (BPS Bioscience #79995) with various amounts of Papain-like protease.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus, COVID-19

Regulatory Status

Research Use Only

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PLPro, His-Tag (SARS-CoV-2) Recombinant

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