Papain-like Protease (SARS-CoV-2) Assay Kit: Deubiquitinase Activity
The Papain-like Protease Assay Kit: Deubiquitinase Activity is designed to measure Papain-like Protease deubiquitinating activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.
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This product has been cited 6 times.
Fluorescent microplate reader capable of reading exc/em=360 nm/460 nm
| Catalog Number |
Component |
Amount |
Storage |
|
| 100735 | Recombinant Papain-like Protease, PLPro | 2 x 2 µg | -80°C | Avoid freeze/ thaw cycles! |
| PLPro Ubiquitinated Substrate (25 µM) | 50 µl | -80°C | ||
| 78039 | PLPro Assay Buffer | 25 ml | -20°C | |
| 10 mM GRL0617 | 20 µl | -80°C | ||
| 0.5 M DTT | 200 µl | -20°C | ||
| 79685 | Black, low binding microtiter plate | 1 | Room Temp |
|
| Plate Sealing Film | 1 | |||
Coronaviruses (CoVs) primarily cause multiple respiratory and intestinal infections in humans and animals. Papain-Like Protease (PLPro), also known as PLP, play an essential role in polypeptide processing during virus replication. PLPro, in addition to being crucial during replication via processing of the viral polyprotein, is proposed to be a key enzyme in the sustained pathogenesis of SARS-CoV. This includes deubiquitination (the removal of ubiquitin) from host-cell proteins. The last enzymatic activity result in the antagonism of the host antiviral innate immune response. As a result, PLpro is an important potential target for antiviral drugs that may inhibit viral replication and weaken dysregulation of signalling cascades in infected cells that may lead to cell death in surrounding, uninfected cells. PLPro inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering with the COVID-19 coronavirus infection.
Ewelina Weglarz-Tomczak, et al., 2020. https://doi.org/10.1101/2020.05.17.100768.
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