CRE/CREB Luciferase Reporter HEK293 Cell Line (cAMP/PKA Signaling Pathway)

Catalog #

60515

$2,070 *

Size: 2 vials

Qty

Bulk/Custom

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Description

The CRE/CREB Luciferase Reporter HEK293 Cell Line (cAMP/PKA Signaling Pathway) is an HEK293 cell line that contains a firefly luciferase reporter under the control of multimerized cAMP response element (CRE), designed to monitor the activity of the cAMP/ PKA signaling pathway. Elevation of intracellular cAMP levels activates cAMP response element binding protein (CREB) to bind to CRE and induces expression of luciferase. This cell line is validated for response to stimulation by forskolin and to treatment with an inhibitor of the cAMP/PKA signaling pathway.

Interested in screening and profiling inhibitors or activators of CRE-dependent signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

This product has been cited 3 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

CRE cell line, CREB cell line, luciferase reporter

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Product Data Gallery

Dose response of CRE/CREB Luciferase Reporter HEK293 Cell Line to forskolin.

Inhibition of forskolin-induced reporter activity by H-89 in CRE/CREB Luciferase Reporter HEK293 Cell Line.

Product Info

Storage and Usage

Citations3

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Materials Required For Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1F	BPS Bioscience #79540

Materials Required for Cellular Assay

Name	Ordering Information
Forskolin: 10 mM in DMSO: activator of cAMP expression	LC Laboratories #F-9929
H-89: 10 mM in DMSO: inhibitor of cAMP/PKA pathway	Enzo Life Sciences #BML-EI196-0005
Assay Medium: Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1F	BPS Bioscience #79540
96-well tissue culture treated white clear-bottom assay plate	Corning #3610
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms. The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. It is the main binding site of cAMP response element binding protein (CREB) and is responsible for its activation. CRE is the target of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. In the cAMP/PKA signaling pathway, CREB is activated via phosphorylation of PKA and binds to CRE with a general motif of 5’-TGACGTCA-3’. Since CRE is a modulator of the cAMP/PKA signaling pathway, it allows the effects of various inhibitors to be studied.

References

Montminy M.R., et al., 1987 Nature 328(6126): 175-178.
Fan Chung K., 2006 Eur. J. Pharmacol. 533(1-3): 110-117.
Malik R., et al., 2008 Appl. Microbiol. Biotechnol. 77 (5): 1167-1173.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1F (BPS Bioscience #79540): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin plus 100 µg/ml Hygromycin B.

Media Required for Functional Cellular Assay
Assay Medium: Thaw Medium 1 (BPS Bioscience #60187)

Instructions for Use

See data sheet for detailed culturing and assay protocol.

Applications

Monitor cAMP/PKA signaling pathway activity.
Screen for compound activity on the cAMP/PKA signaling pathway

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only