CRE/CREB eGFP Reporter Lentivirus

Catalog #

78153

$795 *

Size: 500 µl x 2

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CRE/CREB eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an eGFP gene driven by a multimerized cAMP response element (CRE) located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the cAMP/PKA signaling pathway in the target cells can be monitored by examining eGFP expression.

Figure 1. Schematic of the lenti-vector used to generate the CRE/CREB eGFP reporter lentivirus

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Synonyms

CRE Lentivirus, CREB Lentivirus, CRE Lenti, CREB Lenti, eGFP Lentivirus, eGFP Lenti, Reporter Lentivirus

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Product Info

Storage and Usage

Citations

Species

Replication Incompetent HIV

Supplied As

Two vials (500 µl x 2) of lentivirus at a titer ≥5 x 10⁶ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Information
Forskolin	Sigma #F3917
Thaw Medium 9	BPS Bioscience #79665
Polybrene	Millipore #TR-1003-G
96-well tissue culture treated white clear-bottom assay plate	Corning #3610

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C.

Instructions for Use

See datasheet for details.

Applications

Screen for activators or inhibitors of cAMP/PKA signaling pathway in the transduced target cells
Generation of CRE/CREB eGFP reporter stable cell line

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Biosafety
None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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CRE/CREB eGFP Reporter Lentivirus

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