CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA Signaling Pathway)
The CRE/CREB Luciferase Reporter CHO Cell Line (cAMP/PKA Signaling Pathway) contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into CHO cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. This cell line is validated for response to Forskolin.
Interested in screening and profiling inhibitors or activators of the cAMP pathway without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Media Required for Cell Culture
| Name | Ordering Information |
| Thaw Medium 3 | BPS Bioscience #60186 |
| Growth Medium 3 | BPS Bioscience #79539 |
Materials Required for Cellular Assay
| Name | Ordering Information |
| Assay Medium: Thaw Medium 3 | BPS Bioscience #60186 |
| Growth Medium 3 | BPS Bioscience #79539 |
| Forskolin | Sigma #F3917 |
| 96-well tissue culture treated white clear-bottom assay plate | Corning #3610 |
| ONE-Step™ Luciferase Assay System | BPS Bioscience #60690 |
| Luminometer |
The cell line has been screened to confirm the absence of Mycoplasma species.
Cyclic adenosine monophosphate (cAMP) is a second messenger involved in cell signaling that regulates various physiological and pathological processes. cAMP regulates the transcription of target genes by activating protein kinase A (PKA) and the transcription factor cAMP response element-binding protein (CREB), a downstream effector. CRE is the target of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors), and neurotrophins. In the cAMP/PKA signaling pathway, CREB is activated via PKA-mediated phosphorylation and binds to CRE with a general motif of 5’-TGACGTCA-3’. The cAMP/PKA/CREB signaling pathway has both tumor-suppressive and tumor-promoting effects in cancer cells and can be useful in studying cancer signaling pathways.
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