Claudin-18 Isoform 2, FLAG-Tag Recombinant

Only %1 left

Catalog #

101570

As low as $295 *

Cat # Size Price*

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant human Claudin-18, Isoform 2 (CLDN 18), full length, encompassing amino acids 1-261(end). This construct contains a C-terminal Flag-tag. The recombinant protein was affinity purified.

●

Synonyms

Claudin-18, CLDN18, UNQ778/PRO1572

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CLDN 18.2: CLDN 18.2 Antibody Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Construct

Claudin-18 Isoform 2 (1-261(end)-FLAG)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM HEPES, pH 8, 500 mM NaCl, 10% glycerol, 0.1% DDM, 0.01% CHS, and 100 µg/ml FLAG peptide

29 kDa

Amino Acids

1-261(end)

Genbank #

NM_001002026

UniProt #

P56856

Tag(s)

C-terminal FLAG-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

The protein was validated by measuring its binding to Anti-Claudin-18 Isoform 2 IgG Antibody (BPS Bioscience #101564) in biochemical binding (ELISA) assay. The Claudin-18 Isoform 2 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified Anti-Claudin-18 Isoform 2 IgG Antibody (BPS Bioscience #101564), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with HRP Colorimetric Substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only