Anti-Claudin-18 Isoform 2 Antibody, FITC-Labeled

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Catalog #

101866

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Description

This anti-Claudin-18 isoform 2 (CLDN18.2) antibody is a purified recombinant antibody, which is labeled with fluorescein isothiocyanate (FITC). This antibody has been tested by flow cytometry for specific binding to human CLDN18.2 on the surface of CLDN18.2 CHO Recombinant Cell Line (BPS Bioscience #78533).

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Synonyms

CLDN18, UNQ778, PRO1572, Claudin 18.2

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Product Data Gallery

Cell surface expression of CLDN18.2 in CLDN18.2 CHO Cell Line.

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatant.

Format

Aqueous buffer solution.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain a protein stabilizer.

UniProt #

P56856

Label

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein and has excitation and emission spectrum peak wavelengths of approximately 495 nm and 519 nm, causing it to fluoresce green.

Background

Claudins are integral membrane proteins and components of tight junctions. The claudin-18 gene has two splice variants: claudin-18.1, which is found primarily in the lung, and claudin-18.2, which is expressed exclusively in the stomach. Claudin-18.2 is highly expressed in gastric and pancreatic adenocarcinoma and may be involved in tumor development and progression. It is an ideal target for therapy and effort has been put into the development of monoclonal antibodies against claudin 18.2, such as Zolbetuximab. Studies of claudin-18.2 function and expression patterns will continue to broaden our knowledge and application in cancer therapy.

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million CLDN18.2 CHO cells (BPS Bioscience #78533) were blocked and stained with 1 µg of anti-CLDN18.2 antibody, FITC-labeled (BPS Bioscience #101866) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CLDN18.2 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis represents the cell count, while X-axis shows FITC intensity.

Applications

Useful for studying expression of CLDN18.2 on the cell surface by flow cytometry. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human CLDN 18.2. It has not been tested on other species.

Warnings

Do not freeze. Protect from light.

Scientific Category

Cancer Therapy

Regulatory Status

Research Use Only

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Anti-Claudin-18 Isoform 2 Antibody, FITC-Labeled

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