Claudin-18 Isoform 2 CHO Cell Line (High, Medium, or Low Expression)

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Catalog #

78533

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Description

Recombinant clonal CHO cell line stably expressing full length human claudin 18.2 (claudin 18 isoform 2, CLDN 18.2, gene accession number NM_001002026.3). The stable clonal cell line was selected for different levels of Claudin 18.2 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

CLDN18, UNQ778, PRO1572, Claudin 18.2

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Product Data Gallery

Cell surface expression of Claudin 18.2 in Claudin 18.2 CHO Cell Line

Activation of NFAT Luciferase Reporter Jurkat cells in co-culture with Claudin 18.2 CHO cells

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3D	BPS Bioscience #79539

UniProt #

P56856

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Claudins are integral membrane proteins and components of tight junction strands which serve as a physical
barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or
endothelial cell sheets. The claudin-18 gene has two splice variants: claudin-18.1, which is found primarily in the
lung, and claudin-18.2, which is expressed exclusively in the stomach. Claudin-18.2 is highly expressed in gastric
and pancreatic adenocarcinoma and may be involved in tumor development and progression. Due to the
morphological changes and epithelial-mesenchymal transition occurring upon cancer progression, the
extracellular loops of claudin 18.2 become exposed and available for antibody binding. These biological
characteristics suggest that it is an ideal target for therapy and have led to the further development of monoclonal
antibodies against claudin 18.2, such as Zolbetuximab.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience #60186): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin.

Growth Medium 3D (BPS Bioscience #79539): F-12K medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml of Geneticin.

Applications

Test antibodies against Claudin 18.2 and anti-Claudin 18.2 CAR T cells for immunotherapy research and drug discovery.
Screen inhibitors or neutralizing antibodies against Claudin 18.2

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only