Anti-Claudin-18 Isoform2-Anti-CD3 IgG Bispecific Antibody

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Catalog #

101541

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Description

Anti-Claudin-18 isoform2 (CLDN18.2)-Anti-CD3 IgG format bispecific antibody is a purified recombinant human bispecific antibody with T cell Engager. This bispecific antibody has been tested for specific activity in the functional reporter assay using NFAT-luc reporter Jurkat cell line (BPS Bioscience #60621) in the presence of CLDN18.2-CHO cells (BPS Bioscience #78533-H) that overexpress CLDN18.2.

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Synonyms

CLDN18, UNQ778, PRO1572, Claudin 18.2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Activation of NFAT-Jurkat Reporter cells by Anti CLDN 18.2 Anti-CD3 IgG in the presence of CLDN18.2-CHO cells

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Host Species/Expression System

Heavy chain (HC) and Light chain (LC) co-expressed in HEK293

Purity

≥90%

Purification

Protein A affinity

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Total: 150 kDa (Heavy Chain: 50 kDa; Light Chain: 24 kDa)

UniProt #

P56856

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

Experimental design and assay protocol for measuring anti-CLDN18.2-anti-CD3 functional activity using NFAT-luc reporter Jurkat cell line:

Jurkat effector cells with endogenous TCR/CD3 and transfected reporter NFAT-luc (BPS Bioscience #60621) are incubated with increasing concentrations of anti-CLDN18.2 x anti-CD3 bispecific antibody in the presence of CLDN18.2-CHO cells (BPS Bioscience #78533-H) or CHO cells (ATCC #CCL61™).

1. Seed CHO and CLDN18.2-CHO cells at 30,000 cells/well and allow a few hours for the cells to attach in a 96-well clear bottom white plate.

2. Seed Jurkat cells at 30,000 cells/well.

3. Add the bispecific antibody at a recommended dilution range of 100 fM-100 nM. The bispecific antibody simultaneously binds to TCR/CD3 on the NFAT-luc Jurkat reporter cells and tumor antigen CLDN18.2 on CLDN18.2-CHO cells.

4. After 16 hours the luciferase activity is measured using ONE-Step™ Luciferase Assay (BPS Bioscience #60690) per recommended protocol. The bispecific antibody interaction stimulates NFAT-luciferase activity.

Applications

This product is for research use only. It is not suitable for human diagnostic or therapeutic use. The anti-CLDN18.2-anti-CD3 IgG format bispecific antibody can be used for studying CLDN18.2+ cancer cell-mediated T cell activation, using either primary T cells or reporter cell lines such as NFAT-luc-Jurkat cells (BPS Bioscience #60621).

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Anti-Claudin-18 Isoform2-Anti-CD3 IgG Bispecific Antibody

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