Anti-Claudin-18 Isoform 2 IgG Antibody

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Catalog #

101564

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Description

Anti-Claudin-18 isoform 2 (CLDN18.2) IgG1 monoclonal antibody is a purified recombinant antibody which recognizes human CLDN18.2 antigen. This antibody has been tested for specific binding affinity to purified human CLDN18.2 protein.

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Synonyms

CLDN18, UNQ778, PRO1572, Claudin 18.2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

CLDN18.2: Anti-CLDN18.2 Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Total: 150 kDa; Light Chain: 24 kDa; Heavy Chain: 51 kDa

UniProt #

P56856

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

Functional validation: The antibody was validated by measuring its binding to CLDN18.2 antigen in ELISA. The CLDN18.2 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-CLDN18.2 antibody (BPS Bioscience #101564), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with Colorimetric HRP substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Applications

This product is for research use only. It is not suitable for human diagnostic or therapeutic use.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

101564 Lot 220907

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Anti-Claudin-18 Isoform 2 IgG Antibody

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