Anti-Claudin-18 Isoform 2 IgG Antibody, Biotin-Labeled

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Catalog #

101565

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Description

Biotinylated anti-Claudin-18 isoform 2 (CLDN18.2) IgG1 monoclonal antibody is a purified recombinant antibody that recognizes human CLDN18.2 antigen. This antibody has been tested for specific binding to purified human CLDN18.2 protein.

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Synonyms

CLDN18, UNQ778, PRO1572, Claudin 18.2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CLDN18.2: Anti-CLDN18.2-Biotin Binding Assay

Cell surface staining of CLDN18.2 in CLDN18.2 CHO Cell Line, measured by flow cytometry

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

Heavy chain (HC) and Light chain (LC) co-expressed in HEK293

Purity

≥90%

Purification

Protein A affinity

Format

Aqueous buffer solution

Formulation

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Total: 150 kDa; Light Chain: 24 kDa; Heavy Chain: 51 kDa

UniProt #

P56856

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Storage/Stability

At least 12 months at -80°C.

Assay Conditions

Functional validation: The antibody was validated by measuring anti-CLDN18.2 binding to CLDN18.2 antigen in ELISA. The CLDN18.2 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS detergent (dodecyl-β-D-maltoside (DDM) 0.05%, cholesteryl hemisuccinate (CHS) 0.01%) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified biotinylated anti-CLDN18.2 antibody (BPS Bioscience #101565), serially diluted in Blocking Buffer 2 with DDM/CHS detergent, was added for 60 minutes at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP, washed, and incubated with colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Flow cytometry validation: One million cells were stained with 1 µg of biotinylated anti-CLDN18.2 IgG for 30 minutes on ice, washed three times, stained with Streptavidin PE Conjugate (ThermoFisher #12-4317-87) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CLDN18.2 CHO cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.

Applications

This product is for research use only. It is not suitable for human diagnostic or therapeutic use.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

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Datasheets/Protocols

101565 Lot 220908

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Anti-Claudin-18 Isoform 2 IgG Antibody, Biotin-Labeled

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