CD47 - HEK293 Cell Line

Catalog #: 71249
Size: 2 vials
CD47 (also known as Rh-associated protein, GP42, integrin-associated protein (IAP), and neurophilin,) is an immunoglobulin-like protein that interacts with its receptor, Signal-regulatory protein alpha (SIRPα), on macrophages. This binding interaction regulates transmigration, oxidative burst cytokine production, and phagocytosis, creating a “don’t eat me” signal. CD47 is ubiquitously expressed on the surface of normal cells, but is overexpressed in numerous cancer cells, where it is thought to contribute to the resistance of tumors to phagocyte-dependent clearance.
Recombinant HEK293 cell line over-expressing full length human CD47 (NM_198793.2). Surface expression is confirmed by flow cytometry.
Rh-associated protein, GP42, integrin-associated protein (IAP), neurophilin
Host Cell line:
Human Embryonic Kidney cell line (HEK293). Adherent epithelial cells.
Growth Media:

Thaw Medium 1 (BPS, #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS (Invitrogen, #26140-079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Growth Medium 1F (BPS, #79540): Thaw Medium 1 (BPS, #60187) plus 100 µg/ml Hygromycin B (Thermo Fisher, #10687010).

Each vial contains ~3 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Ideal for protein binding analyses and studying SIRPα receptor activity.
Data shown is lot-specific. Contact us for specific information on other lots.

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